Biochem/physiol Actions

Cell permeable: yes

General description

A cell-permeable triphenylphosphonium compound that exhibits selective toxicity towards cancer cells and inhibits their proliferation (IC₅₀ = 400, 500, 800 nM and 1.1. µM for HPAC, MIA PaCa-2, BxPC-3, and PANC-1 pancreatic cancer cells, respectively). Arrests cell cycle at G0/G1 phase of the cell cycle in a time- and dose-dependent manner. It accumulates in mitochondria and induces mitochondrial and cytosolic accumulation of reactive oxygen species. Induces apoptosis by activating caspase-8 and 7 and by lowering the levels of Bcl-2 and survivin in cancer cells. Reduces autophagy as evidenced by a reduction in beclin-1 and increase in LC3B-II and p62 levels (at ~20 µM). Also shown to diminish Src phosphorylation (Tyr416) correspondingly reduce in Ser576 and Tyr861 phosphorylation in focal adhesion kinase (FAK).

A cell-permeable triphenylphosphonium compound that exhibits selective toxicity towards cancer cells and inhibits their proliferation (IC₅₀ = 400, 500, 800 nM and 1.1. µM for HPAC, MIA PaCa-2, BxPC-3, and PANC-1 pancreatic cancer cells, respectively). Arrests cell cycle at G0/G1 phase of the cell cycle in a time- and dose-dependent manner. It accumulates in mitochondria and induces mitochondrial and cytosolic accumulation of reactive oxygen species. Induces apoptosis by activating caspase-8 and 7 and by lowering the levels of Bcl-2 and survivin in cancer cells. Reduces autophagy as evidenced by a reduction in beclin-1 and increase in LC3B-II and p62 levels (at ~20 µM). Also shown to diminish Src phosphorylation (Tyr416) correspondingly reduce in Ser576 and Tyr861 phosphorylation in focal adhesion kinase (FAK).Please note that the molecular weight for this compound is batch-specific due to variable water content.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Millard, M., et al. 2013. PLos One.5, e13131.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)